ProteoRed - Instituto Nacional de Protemica Sept 7th, 2010 
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Node 1



Centro Nacional de Biotecnología
Web site: http://proteo.cnb.csic.es/
 
Description
The main objective of the Proteomics Facility of the Centro Nacional de Biotecnologia-CSIC (CNB-CSIC) is to guarantee the technological support to permit to the research groups located at the CNB-CSIC and, in a broader sense, to the scientific community the analysis of the proteome.
Services
  Protein and Peptide Molecular Weight Analysis
    Molecular weight analysis by MS-MALDI TOF
    Molecular weight analysis by ESI by MS-QTOF or MS-ION TRAP
    Molecular weight analysis by MS-SELDI TOF
    Protein Profile Analysis using MS-SELDI-TOF
  Protein Identification
    Peptide Mass Fingerprinting MS - MS/MS by MALDI TOF/TOF
    Protein Identification by LC-MS/MS (<5 Proteins)
    Protein Identification by LC-MS/MS (<50 proteins)
    Protein Identification by LC/MS/MS (>50 proteins)
    Protein Identification by LC-MALDI TOF/TOF
    Protein Identification by 2D LC-MS/MS (2 - 5 fractions)
    Protein Identificacion by 2D LC-MS/MS (>5 fractions)
    Protein Identification by nESI-MS/MS (off-line)
  Characterization and protein modifications
    Identification of post-translational Modifications
  Sequencing
    De novo sequencing
  Electrophoresis
    1D Electrophoresis SDS-PAGE
    2D Electrophoresis
  Differential Proteomics
    2D-DIGE
    Quantitative proteomics
    Image Analysis
  Peptide Synthesis
    Peptide Synthesis
    Peptide Arrays up 360 deca-/dodeca peptides
    Peptide antigen design
    Coupling to carriers (KLH, OVA, BSA)
    Antibody purification
  Staining
    Silver Gel Staining
    Fluorescence Staining
  Bioinformatics tools
    Image Analysis Tools
    Functional Data Analysis and Retrieval
    Genomic Analysis for Rapid Biological ANnotation (GARBAN)
  Gluten Analysis
    Quantification by sandwich R5 ELISA
    Quantification by competitive R5 ELISA
    Identification and Characterization - Western Blot R5
    Identification and Characterization - Real Time PCR
    Identification and Characterization - Mass Spec. MALDI-TOF
  Other Services
    Biacore
    Gel Scanning
 
Person in charge and contacts
Person in charge:  Juan Pablo Albar Ramírez - Lab Manager CNB
Staff:  
2D/DIGE:  Marisol Fernández
Bioinformatics:  Alberto Medina Auñón
ESI:  Alberto Paradela Elizalde
ESI:  Rosa Ana Navajas
MALDI, SELDI-TOF:  Sergio Ciordia
MALDI-TOF/TOF:  Silvia Juárez
Peptide synthesis:  Fernando Roncal
 
Other contacts:  Salvador Martínez de Bartolomé Izquierdo
 




Centro de Biología Molecular Severo Ochoa
Web site: http://www.cbm.uam.es/servicios/proteomica.htm
 
Description
In 1993 the Protein Chemistry Facility was created in C.B.M.S.O. The trajectory of the Proteomics Facility has been parallel to the evolution of the scientific community in the study of proteins and its objectives are: -To offer support and technical advice to the investigator with appropriate methods to prepare the sample and design of the appropriate work-flow for each objective. -To apply all the techniques available in the laboratory for the analysis of proteins. Our Service has been used by numerous research projects in a wide range of scientific fields. Although it is a service widely used by researchers from different regions, the most frequent customers come from the C.B.M.S.O.
Services
  Protein and Peptide Molecular Weight Analysis
    Molecular weight analysis by MS-MALDI TOF
  Protein Identification
    Peptide Mass Fingerprinting by MS-MALDI TOF
    Peptide Mass Fingerprinting by MS-MALDI-TOF employing Ultra Fast Trypsin Digestion
    Protein Identification by LC-MS/MS (<5 Proteins)
    Protein Identification by LC-MS/MS (<50 proteins)
    Protein Identification by nESI-MS/MS (off-line)
  Characterization and protein modifications
    Identification of post-translational Modifications
  Sequencing
    De novo sequencing
  Electrophoresis
    1D Electrophoresis IEF
    1D Electrophoresis SDS-PAGE
    2D Electrophoresis
  Staining
    Coomassie Gel Staining
    Fluorescence Staining
  Sample Preparation
    Desalting and concentration by Zip-Tip
    Sample precipitation
  Other Services
    Gel Scanning
 
Person in charge and contacts
Person in charge:  Anabel Marina - Lab Manager CBMSO
Staff:  
2DE:  Esperanza Morato
ESI-IT:  Yolanda Nuñez
ESI-IT:  Mercedes Del Valle
 
 




Parque Científico de Madrid – Universidad Complutense de Madrid
Web site: http://www.ucm.es/proteomica
 
Description
The aim of the Proteomics Facility UCM-PCM is to provide the scientific community with the most suitable tools for the proteomic approach. The Center offers not only different proteomic services but also courses and workshops.

Services
  Protein and Peptide Molecular Weight Analysis
    Molecular weight analysis by MS-MALDI TOF
  Protein Identification
    Peptide Mass Fingerprinting by MS-MALDI TOF
    Peptide Mass Fingerprinting MS - MS/MS by MALDI TOF/TOF
    Protein Identification by LC-MS/MS (<5 Proteins)
    Protein Identification by LC-MS/MS (<50 proteins)
    Protein Identification by LC/MS/MS (>50 proteins)
    Protein Identification by LC-MALDI TOF/TOF
    Protein Identification by 2D LC-MS/MS (2 - 5 fractions)
    Protein Identificacion by 2D LC-MS/MS (>5 fractions)
    Protein Identification by nESI-MS/MS (off-line)
  Characterization and protein modifications
    Identification of post-translational Modifications
  Sequencing
    Peptide Sequencing by MALDI TOF/TOF
    De novo sequencing
  Electrophoresis
    1D Electrophoresis IEF
    1D Electrophoresis SDS-PAGE
    2D Electrophoresis
  Differential Proteomics
    2D-DIGE
    Labeling for ITRAQ
    Other quantitative mass spectrometry
    Quantitative proteomics
    Image Analysis
  Staining
    Fluorescence Staining
  Sample Preparation
    Desalting and concentration by Zip-Tip
    Sample precipitation
  Other Services
    Gel Scanning
    Electroblotting
    Other services
 
Person in charge and contacts
Person in charge:  Concha Gil García - Lab Manager PCM/UCM
Staff:  
MALDI-TOF/TOF:  Lola Gutiérrez Blázquez
Q-TOF:  M.Luisa Hernáez Sánchez
 
Other contacts:  Montserrat Martínez-Gomariz
Pilar Ximénez de Embún
Felipe Clemente Velarde
 




Centro de Investigaciones Biológicas *
Web site: http://www.cib.csic.es/es/servicio.php?iddepartamento=29
 
Description
* Associated center
The Proteomics and Genomics Facility at CIB is open to all researchers on a fee-for-service basis. User fees will be adjusted depending on the customer institution and to keep costs competitive and affordable for the scientific community.
Services
  Protein and Peptide Molecular Weight Analysis
    Molecular weight analysis by MS-MALDI TOF
  Protein Identification
    Peptide Mass Fingerprinting by MS-MALDI TOF
    Peptide Mass Fingerprinting MS - MS/MS by MALDI TOF/TOF
    Protein Identification by LC-MS/MS (<5 Proteins)
  Electrophoresis
    1D Electrophoresis SDS-PAGE
    2D Electrophoresis
  Staining
    Silver Gel Staining
    Coomassie Gel Staining
    Fluorescence Staining
  Sample Preparation
    Desalting and concentration by Zip-Tip
    Sample precipitation
  Other Services
    Gel Scanning
 
Person in charge and contacts
Person in charge:  Ignacio Casal
 
Other contacts:  Vivian de los Ríos Benítez
Francisco García Tabares
Andrés Fernández Gil
 


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