Jul 30th, 2010  General Structure Nodes Proteomics Facilities Management Board Scientific Advisory Board User Committee Coming Events Past Events Samples - PME 5 Back to Experiment Description page   Samples:   Each participant laboratory will receive two protein mixture samples, labeled A and B, containing each 100 µg of total protein.   Samples are in solution, in 20mM Tris.HCl, 50 mM NaCl, and are sent frozen.   Concentration of both samples is 6.6 mg/mL   New samples were sent on January 26th: Samples are identical to the previous ones (100 micrograms of each protein mixture A and B), except that this time they are dissolved in 6M Urea /1% CHAPS, at 6 micrograms/microliter concentration.                       Samples contain:           A mixture of around 150 E. Coli proteins (identical in each sample). This mixture has been prepared by fractionation of the cytoplasmatic proteome of E.Coli. It contains soluble proteins, of a wide range of pI and Mw.           Four spiked mammalian proteins:           CYC_HORSE (Cytochrome C, Mw 12362), added at the ~ 1 pmol/ 1 mg total protein level.     MYG_HORSE (Apomyoglobin, Mw 16952), at ~ 200 fmol / 1 mg total protein     ALDOA_RABIT (Aldolase, Mw 39212), at ~ 25 fmol / 1 mg total protein     ALBU_BOVIN (Serum albumin, Mw 66430), at ~ 1 fmol / 1 mg total protein           These four proteins have been spiked in different amounts in samples A and B, with ratios ranging from 1.5:1 to 5:1 between the two samples.                     Back to Experiment Description page   Working Group 1 | Working Group 2 | Working Group 3 | Working Group 4 Working Group 5 | Working Group 6 | Working Group 7 @2009 ProteoRed. All rights reserved. Credits