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Experiment reporting: |
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As we did in previous editions, all experiments performed in this ProteoRed Multicenter Experiment will be reported using the MIAPE generator tool. |
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The documents will be stored in a public project called "ProteoRed multicenter assays 2010 (2)" (ID:470) |
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Detailed information here (PDF) |
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Main considerations: |
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- there is a set of templates that were created after the MIAPE meeting that we had in Miraflores that can help you to know how to organize the information.
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- the labs that have MASCOT v2.3 (they can export the searches to mzIdentML file), can create the MIAPE MSI document in an automatic way using a JAVA application (here).
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- Francesc have asked to add some extra information to the MIAPE MS document, that you can add in the Additional Information section:
- Digestion protocol:
- Denaturant added and initial concentration
- Reduction reagent used , concentration, temperature, time of reaction
- Alkylation reagent uses, concentration, temperature, time of reaction
- Buffer for digestion, denaturant and concentration
- Ratio w/w trypsin used
- Temperature / time digestion
- Chromatography:
- Trap column used
- Column used, lenght of column
- Gradient (initial-final % ACN), time
- Amount of sample
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If you have any problem with the tools, or you don't know your user/password to access to your projects, please contact to smartinez[]proteored.org |
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