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Goals: The primary goals were to provide each participating laboratory with an opportunity to evaluate its capabilities and approaches with regard to detecting phosphopeptides and identifying sites of phosphorylation. In the analysis of the study sample, participants were encouraged to use one or more steps of phosphopeptide enrichment. Most importantly, this study represented the first step in developing a phosphopeptide standard that more closely resembles a “real life” sample. Laboratories requesting samples received a mixture containing a tryptic digest of up to 6 proteins, all of which will contained one or more phosphorylated residues. The peptides were present in sufficient quantities (10 pmoles each) to permit utilization of a variety of strategies.
Figure 1: ProteoRed members participating in the sPRG2010 contest
ProteoRed participating laboratories:
Figure 2: Summary of the results obtained by one of the ProteoRed participants.
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