Working Group 1
Technological development and Standardization of protocols
Person in charge: Alberto Paradela
Group: Anabel Marina, Bernardo Celda, Cristina Chiva, David Andreu, Eliandre de Oliveira, Félix Elortza, Francesc Canals, Ignacio Casal, jesús M. Arizmendi, Joan López, Joaquín Abián, Joaquín Arribas, José A. Bárcena, Josep M. Estanyol, Juan F. Santarén, Juan Pablo Albar, Laura Sesma, Lidia Borrel, Manuel S. del Pino, Maria Luisa Hernando, Marina Gay, Miriam Fernández, Nieves Ibarrola, Oriol Bachs, Roque Bru, Samuel Ogueta, Silvia Bronsoms, Valentín Ceña
The specific objetives of the technological develoment are:
Allow a continuous improvement in the throughput and the performance of Proteomics Facilities of the Network.
Provide access for researchers to state-of-the-art Proteomics techniques.
Support new technical and scientific personnel contracted by ProteoRed nodes in the performance of proteomics technologies.
Coordinate and define the strategy for the acquisition of new equipment in order to optimize its utilization by research projects in Proteomics.
The specific objetives of the standardization of protocols are:
Collect and evaluate proteomics protocols used by ProtoeRed members to improve the quality of the data generated in their laboratories.
Define the minimum quality criteria that should be fulfilled by ProteoRed members.
Study the proteomics protocols and standards internationally accepted in order to distribute them to ProteoRed members and users.
Promote activities among ProteoRed members in order to guarantee the quality of the services.
Encourage the exchange of technical information among ProteoRed members.
Finally, one of the main goals will be the compilation and discussion of the new experimental approaches continuously being developed and, eventually, to encourage their use by ProteoRed for the improvement of service quality by all the members.
Summary of WG1 activities
The following is a summary of the 2005-2010 WG1 activities in which most of ProteoRed lab members have taken part. WG1 objectives clearly fit in both ABRF (http://www.abrf.org) PRG (Proteomics Research Group) and sPRG (standard Proteomics Research Group) activities and, thus, WG1 have encouraged the participation of ProteoRed members in the contests organized by the ABRF. This strategy has helped us to evaluate proteomics capabilities of ProteoRed members, both in terms of knowledge and scientific equipment, in an international context. Participation of ProteoRed members in the ABRF contests has been very important both in quantitative and qualitative aspects. Some examples are highlighted in the following figure:
(Red/orange boxes indicate the activities/contests in which ProteoRed members have participated)
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ABRF-sPRG2006 Study: A Proteomics Standard |
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ProteoRed 2006 (UPS Sigma Aldrich) |
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ABRF-sPRG2007 Study: Development and evaluation of a Phosphoprotein Standard |
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ABRF PRG2008: Qualitative Proteomics Study-Identifying differences in Primary Structure. |
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ABRF sPRG2009: Development of Quantitative Proteomics Standards |
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ABRF PRG2009: Relative Protein Quantification in a Clinical Matrix |
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ABRF sPRG2010: Multilaboratory evaluation of a Phosphopeptide standard for proteomics |
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ABRF PRG2010: Tackling Unforeseen problems in otherwise straightforward Proteomics analysis. |
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Working Group 1 | Working Group 2 | Working Group 3 | Working Group 4
Working Group 5 | Working Group 6 | Working Group 7
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