ProteoRed - Instituto Nacional de Proteómica 7/25/2008 

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Working Group 1

Technological development and Standardization of protocols

Person in charge: Juan Pablo Albar

Group: Alberto Paradela, Anabel Marina, Bernardo Celda, Cristina Chiva, David Andreu, Eliandre de Oliveira, Félix Elortza, Francesc Canals, Ignacio Casal, jesús M. Arizmendi, Joan López, Joaquín Abián, Joaquín Arribas, José A. Bárcena, Josep M. Estanyol, Juan F. Santarén, Laura Sesma, Lidia Borrel, Manuel S. del Pino, Maria Luisa Hernando, Marina Gay, Miriam Fernández, Nieves Ibarrola, Oriol Bachs, Roque Bru, Samuel Ogueta, Silvia Bronsoms, Valentín Ceña

The specific objetives of the technological develoment are:

Allow a continuous improvement in the throughput and the performance of Proteomics Facilities of the Network.

Provide access for researchers to state-of-the-art Proteomics techniques.

Support new technical and scientific personnel contracted by ProteoRed nodes in the performance of proteomics technologies.

Coordinate and define the strategy for the acquisition of new equipment in order to optimize its utilization by research projects in Proteomics.

The specific objetives of the standardization of protocols are:

Collect and evaluate proteomics protocols used by ProtoeRed members to improve the quality of the data generated in their laboratories.

Define the minimum quality criteria that should be fulfilled by ProteoRed members.

Study the proteomics protocols and standards internationally accepted in order to distribute them to ProteoRed members and users.

Promote activities among ProteoRed members in order to guarantee the quality of the services.

Encourage the exchange of technical information among ProteoRed members.

Finally, one of the main goals will be the compilation and discussion of the new experimental approaches continuously being developed and, eventually, to encourage their use by ProteoRed for the improvement of service quality by all the members.

Current activities:

To achieve the main objetive of this working group, to stimulate the comparison of experimental protocols and data obteined through the analysis of reference samples whith the aim of standardize the multiple methodologic and technologic aproximations, the labs of ProteoRed have been encourage to participate at the ABRF 2006 Proteomics Standar Research Group (sPRG) study.

The objective of this study, is the identification of the mayor number of proteins from a sample of fifty proteins given. This mixture of proteins is an excelent reference standard for the comparison of protocols and methodologies.

We expect to take out conclusions which permit to improve and develop the analysis capability of ProteoRed labs. Once the conclusions are elaborated, some actions will be stablished.

1st WG 1 Meeting will be done on 14-15 march 2006. Some of the points discussed, will be:

     1) Repeat the analysis with different techniques, alternatives to the ones used previously.

     2) Repeat the analysis with the same technological platform, but ussing different experimental approaches.

     3) The diffusion of the new resaults and the conclusion deduced.




Working Group 1 | Working Group 2 | Working Group 3 | Working Group 4
Working Group 5 | Working Group 6

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